Serotype diversity of respiratory human adenoviruses amongst pediatric patients from Western Kenya, 2010-2012
Date
2016Author
Mbui, Fredrick M.
Achilla, Rachel A
Coldren, Rodney L.
Bulimo, Wallace D.
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Background: Respiratory illnesses are common among pediatric patients in Kenya, and many are attributed to viral
causes. However, there is limited knowledge of the diversity of viral etiologies associated with these illnesses.
Objective: To characterize respiratory adenoviruses isolates using serological and molecular approaches.
Methods: A total of 1,879 samples were collected from symptomatic pediatric patients seeking medical care at New
Nyanza Provincial General Hospital during the period of June 2010 to June 2012 and screened for adenoviruses as
well as other respiratory viruses. Sixteen respiratory human adenoviruses (HAdVs) were isolated in Hep2 cell culture
and characterized them using Immunofluorescence Assay, viral DNA amplification, sequencing and phylogenomics.
Results: Phylogenetic characterization of the HAdVs using the hyper variable region 7 of the hexon gene identified
HAdV B and C as the major species associated with respiratory infections during the study period. Amongst these, a
single B-type and four C-type serotypes were identified. The serotype distribution consisted of 31% HAdV B7, 25%
HAdV C1, 25% HAdV C2, 6% HAdV C5, and 13% HAdV C6. Positive selection was observed in the nucleotide
sequences from HAdV B7 and HAdV C5 signaling evolution of these two serotypes.
Conclusion: These finding may be useful to policy makers regarding appropriate strain selection for vaccination in
Keny
Citation
Mbui, Fredrick M., et al. "Serotype Diversity of Respiratory Human Adenoviruses amongst Pediatric Patients from Western Kenya, 2010-2012." African Journal of Pharmacology and Therapeutics 5.3 (2016).Publisher
University of Nairobi
Rights
Attribution-NonCommercial-NoDerivs 3.0 United StatesUsage Rights
http://creativecommons.org/licenses/by-nc-nd/3.0/us/Collections
- Faculty of Health Sciences (FHS) [10387]
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