dc.contributor.author | Schellenberg, John J. | |
dc.contributor.author | Card, Catherine M. | |
dc.contributor.author | Ball, T. Blake | |
dc.contributor.author | Mungai, Jane Njeri | |
dc.contributor.author | Irungu, Erastus | |
dc.contributor.author | Kimani, Joshua | |
dc.contributor.author | Jaoko Walter G. | |
dc.contributor.author | Wachihi, Charles | |
dc.contributor.author | Fowke, Keith R. | |
dc.contributor.author | Plummer, Francis A. | |
dc.date.accessioned | 2013-02-19T05:39:21Z | |
dc.date.issued | 2012 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/22095193 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/10143 | |
dc.description.abstract | Although bacterial vaginosis is a known correlate of HIV infection, no previous studies have investigated whether women defined as HIV-exposed seronegative (HESN) are less likely to have bacterial vaginosis. Little is known about the effects of bacterial vaginosis on systemic immune activation associated with HIV+ serostatus. Cohort-based retrospective analysis of bacterial vaginosis in relation to HESN status, HIV+ serostatus and peripheral T-helper cells, with cross-sectional analysis of bacterial vaginosis in relation to peripheral T-regulatory cells (Tregs). Bacterial vaginosis diagnosis by Gram stain and determination of systemic CD4(+) and CD8(+) T-helper cell frequency by flow cytometry for 3504 vaginal samples from 988 commercial sex workers over 4 years. Tregphenotyping by FoxP3 staining and multiparameter flow cytometry in peripheral blood of 97 women at a single time-point. No differences in bacterial vaginosis diagnosis were observed between HESN and other HIV-negative (HIV-N) controls; however, HIV+ women were more likely to be diagnosed with bacterial vaginosis compared to all HIV-negative women (HESN/HIV-N combined). HIV+ women with bacterial vaginosis had significantly higher CD4(+)/CD8(+) T-helper cell counts and a lower CD4/CD8 ratio, as well as fewer Tregs as a proportion of total T-helper cells, compared to bacterial vaginosis-negative women. The number of bacterial vaginosis diagnoses in this cohort has decreased significantly over time. Bacterial vaginosis is associated with HIV serostatus and shifts in distribution of T-cell subsets. A concomitant reduction in bacterial vaginosis and HIV infections over time suggests that the elucidation of bacterial vaginosis-HIV interactions will be critical to further understanding of HIV pathogenesis and prevention in this high-risk group. | en |
dc.language.iso | en | en |
dc.subject | Bacterial vaginosis, | en |
dc.subject | Commercial sex workers, | en |
dc.subject | HIV resistance, | en |
dc.subject | HIV/AIDS, | en |
dc.subject | Multiparameter flow cytometry, | en |
dc.subject | T-helper cell subsets, | en |
dc.subject | Treg | en |
dc.title | Bacterial vaginosis, HIV serostatus and T-cell subset distribution in a cohort of East African commercial sex workers: retrospective analysis | en |
dc.title.alternative | Retrospective analysis | en |
dc.type | Article | en |