Liquid chromatographic analysis of phenobarbitone, ethosuximide, phenytoin and carbamazepine on a polystyrene-divinyl benzene column
Date
2006Author
Amugune, BK
Thoithi, GN
Kibwage, IO
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
A liquid chromatographic method for the simultaneous assay of four anticonvulsant drugs, phenobarbitone, ethosuximide, phenytoin and carbamazepine on a polystyrene-divinyl benzene column is described. The method was developed by the systematic study of different types of co-polymer materials, type and concentration of organic modifiers, buffer pH and concentration and column temperature. A PLRP-S 100 A 8 um column maintained at 60°C and a mobile phase consisting of acetonitrile-tert-butanol-phosphate buffer (pH 7.6, 0.2 M)-water (25:5:10:60, v/v) were used. The flow rate was 1 ml/min with ultraviolet detection at 220 nm. The method has been validated and used for the analysis of raw materials, finished products and dissolution studies of the drugs
Citation
East and Central African Journal of Pharmaceutical Sciences Vol. 9 (2006) 19 - 25Publisher
Department of Pharmaceutical Chemistry, School of Pharmacy, University of Nairobi
Subject
Liquid chromatography,Co-polymer column,
Phenobarbitone,
Ethosuximide,
Phenytoin,
Carbamazepine
Collections
- Faculty of Health Sciences (FHS) [10378]