dc.contributor.author | Ng'etich, D. K. | |
dc.contributor.author | Bett, R. C. | |
dc.contributor.author | Gachuiri, C. K. | |
dc.contributor.author | Kibegwa, F. M | |
dc.date.accessioned | 2022-04-11T09:27:27Z | |
dc.date.available | 2022-04-11T09:27:27Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Ng'etich, D. K., Bett, R. C., Gachuiri, C. K., & Kibegwa, F. M. (2022). Diversity of Gut Methanogens and Functional Enzymes Associated With Methane Metabolism in Smallholder Dairy Cattle. | en_US |
dc.identifier.uri | https://doi.org/10.21203/rs.3.rs-1120235/v1 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke/handle/11295/160144 | |
dc.description.abstract | Methane is a greenhouse gas with disastrous consequences when released to intolerable levels.
Ruminants produce methane during gut fermentation releasing it through belching and/or flatulence. To
better understand the diversity of methanogens and functional enzymes associated with methane
metabolism in dairy cows, 48 samples; six rumen and 42 dung contents were collected and analyzed
using a shotgun metagenomic approach. The results indicated archaea from 5 phyla, 9 classes, 16
orders, 25 families, 59 genera, and 87 species. Gut sites significantly contributed to the presence and
distribution of various methanogens (P<0.01). The class Methanomicrobia was abundant in the rumen
samples (~ 39%) and in dung (~44%). The most abundant (~17%) methanogen species identified was
Methanocorpusculum labreanum. However, some taxonomic classes were not classified (~ 6% in the
rumen and ~4% in the dung). Furthermore, five functional enzymes: Glycine/Serine hydroxy
methyltransferase, Formylmethanofuran—tetrahydromethanopterin N-formyltransferase, Formate
dehydrogenase, Anaerobic carbon monoxide dehydrogenase and Catalase-peroxidase were associated
with methane metabolism. KO0600 module and Enzyme Commissions (1.11.1.6 & 2.1.2.1) were common
for dung and rumen fluid’s enzymatic pathways. Functional analysis for the enzymes identified were
significant (P<0.05) for 5 metabolism processes. Breeding for tolerable methane emitting dairy cattle for
a sustainable environment should be undertaken | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Research Square | en_US |
dc.subject | Ruminants, species, greenhouse, Enzymes, Kenya, Tanzania | en_US |
dc.title | Diversity of Gut Methanogens and Functional Enzymes Associated With Methane Metabolism in Smallholder Dairy Cattle | en_US |
dc.type | Preprint | en_US |