dc.contributor.author | Terefe, Ermias M | |
dc.contributor.author | Okalebo, Faith A | |
dc.contributor.author | Derese, Solomon | |
dc.contributor.author | Gaber, El-Saber B | |
dc.contributor.author | Amal, Youssef | |
dc.contributor.author | Alorabi, Mohammed | |
dc.contributor.author | Muriuki, Joseph | |
dc.date.accessioned | 2022-06-16T07:00:33Z | |
dc.date.available | 2022-06-16T07:00:33Z | |
dc.date.issued | 2022-02 | |
dc.identifier.citation | Terefe EM, Okalebo FA, Derese S, Batiha GE, Youssef A, Alorabi M, Muriuki J. Cytotoxicity and anti-HIV activities of extracts of the twigs of Croton dichogamus Pax. BMC Complement Med Ther. 2022 Feb 25;22(1):49. doi: 10.1186/s12906-022-03532-1. PMID: 35216601; PMCID: PMC8876114. | en_US |
dc.identifier.uri | https://pubmed.ncbi.nlm.nih.gov/35216601/ | |
dc.identifier.uri | http://erepository.uonbi.ac.ke/handle/11295/161035 | |
dc.description.abstract | Background: Acquired immunodeficiency syndrome (AIDS) is a clinical syndrome resulting from infection with human immunodeficiency virus (HIV), which causes profound immunosuppression. Anti-HIV drugs that are currently available are chemically synthesized and are frequently limited by side effects, the emergence of drug resistance, affordability, and availability, with over 5 million people in the world lacking access to treatment. As a result, to discover new anti-HIV agents, we investigated the effects of Kenyan C. dichogamus extracts on the laboratory-adapted strain HIV-1IIIB in human T-lymphocytic MT-4 cells.
Methods: Four soluble fractions of 1:1 v/v CH2Cl2:MeOH extract of the twigs of C. dichogamus Pax were tested for their replication inhibition activity against the laboratory-adapted strain HIV-1IIIB in the human T-lymphocytic MT-4 cell line. The plant extracts were further evaluated for their cytotoxicity in MT-4 cells using the MTT assay.
Results: The cytotoxicity CC50 values of the methanol and methylene chloride soluble fractions of C. dichogamus were found to be between 19.58 ± 0.79 and 167 ± 0.8 µg/ml, respectively. The hexane, methylene chloride, and methanol soluble fractions of the 1:1 v/v CH2Cl2:MeOH extract of the twigs of C. dichogamus showed inhibition of the HIV-1IIIB laboratory-adapted strain in a virus-infected cell culture antiviral assay. The methanol soluble fraction of the 1:1 v/v CH2Cl2:MeOH extract of the twigs of C. dichogamus showed significant anti-HIV activity by inhibiting more than 90% of viral-induced cytopathic effects with an IC50 value of 0.06 ± 0.01 µg/ml, giving an SI of 318.5.
Conclusion: Based on our findings, the methanol soluble fraction of the 1:1 v/v CH2Cl2:MeOH extract of the twigs of C. dichogamus has shown potential efficacy in inhibiting viral replication and could be considered a promising candidate for further studies. | en_US |
dc.language.iso | en | en_US |
dc.publisher | University of Nairobi | en_US |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
dc.subject | Anti-viral activity; Croton dichroism; Cytotoxicity; HIV. | en_US |
dc.title | Cytotoxicity and Anti-hiv Activities of Extracts of the Twigs of Croton Dichogamus Pax | en_US |
dc.type | Article | en_US |