Occurrence of serum antibodies to Toxoplasma gondii and associated risk factors in donkeys from central Kenya

Abstract

Toxoplasma gondii is an intracellular parasite of zoonotic concern and economic importance in humans and animals, respectively. This study was conducted to determine the occurrence of T. gondii and associated risk factors in domestic donkeys from Kirinyaga and Meru counties in Kenya. Blood samples were collected from 363 randomly selected donkeys for detection of antibodies to T. gondii using a commercial kit ID Screen® Toxoplasmosis Multi-species indirect enzyme-linked immunosorbent assay (ELISA). The data on risk factors were collected by interviewing donkey owners using epidemiological questionnaire. Serum antibodies to T. gondii were detected in 26.4% (95% CI: 22.2-31.3) of the donkeys. The analysis showed that age of donkeys (OR = 2.484, 95% CI: 1.315-4.693; p = 0.005) was associated with increased risk for T. gondii seroprevalence while county of origin of donkeys (OR = 0.182, 95% CI: 0.083-0.400; p = 0.000), residential place of donkeys (OR = 0.301, 95% CI: 0.136-0.665; p = 0.003), rearing chicken (OR = 0.203, 95% CI: 0.064-0.644; p = 0.007), and donkey production system (OR = 0.644, 95% CI: 0.456-0.909; p = 0.012) were associated with reduced risk of T. gondii seroprevalence. This is the first report to provide epidemiological information on T. gondii infection among donkeys in Kenya. The presence of antibodies to T. gondii in donkeys suggests the high potential of transmission to other animals and humans. Regular monitoring and control of T. gondii infection in donkeys were recommended in the study area.