dc.contributor.author | Yaffe, Zak A | |
dc.contributor.author | Ding, Shilei | |
dc.contributor.author | Sung, Kevin | |
dc.contributor.author | Chohan, Vrasha | |
dc.contributor.author | Marchitto, Lorie | |
dc.contributor.author | Doepker, Laura | |
dc.contributor.author | Ralph, Duncan | |
dc.contributor.author | Nduati, Ruth | |
dc.contributor.author | Matsen, Frederick A | |
dc.contributor.author | Finzi, Andrés | |
dc.contributor.author | Overbaugh, Julie | |
dc.date.accessioned | 2023-10-30T08:25:36Z | |
dc.date.available | 2023-10-30T08:25:36Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Yaffe ZA, Ding S, Sung K, Chohan V, Marchitto L, Doepker L, Ralph D, Nduati R, Matsen FA 4th, Finzi A, Overbaugh J. Reconstruction of a polyclonal ADCC antibody repertoire from an HIV-1 non-transmitting mother. iScience. 2023 Apr 26;26(5):106762. doi: 10.1016/j.isci.2023.106762. PMID: 37216090; PMCID: PMC10196594. | en_US |
dc.identifier.uri | https://pubmed.ncbi.nlm.nih.gov/37216090/ | |
dc.identifier.uri | http://erepository.uonbi.ac.ke/handle/11295/163835 | |
dc.description.abstract | Human natural history and vaccine studies support a protective role of antibody dependent cellular cytotoxicity (ADCC) activity against many infectious diseases. One setting where this has consistently been observed is in HIV-1 vertical transmission, where passively acquired ADCC activity in HIV-exposed infants has correlated with reduced acquisition risk and reduced pathogenesis in HIV+ infants. However, the characteristics of HIV-specific antibodies comprising a maternal plasma ADCC response are not well understood. Here, we reconstructed monoclonal antibodies (mAbs) from memory B cells from late pregnancy in mother MG540, who did not transmit HIV to her infant despite several high-risk factors. Twenty mAbs representing 14 clonal families were reconstructed, which mediated ADCC and recognized multiple HIV Envelope epitopes. In experiments using Fc-defective variants, only combinations of several mAbs accounted for the majority of plasma ADCC of MG540 and her infant. We present these mAbs as evidence of a polyclonal repertoire with potent HIV-directed ADCC activity. | en_US |
dc.language.iso | en | en_US |
dc.publisher | University of Nairobi | en_US |
dc.subject | Immunology; Pediatrics; Virology. | en_US |
dc.title | Reconstruction of a polyclonal ADCC antibody repertoire from an HIV-1 non-transmitting mother | en_US |
dc.type | Article | en_US |