Toxicological assessment of a tsetse fly repellent (Patent No.Ke 00185,2004) on the health of exposed animals.

Abstract

Tsetse and trypanosomosis have been extensively studied and an array of vector and disease control measures have been developed and applied with varying degrees of success. Current trypanosomosis control strategies rely on use of trypanocidal drugs, breeding of trypanotolerant cattle and tsetse control or eradication. Since none of these control options has proved to be a fully viable solution to the control of trypanosomosis, search for more tsetse fly and trypanosomosis control measures continues. A synthetic tsetse fly repellent (patent No. Ke 00185, 2004; Saini and Hassanali), was identified in 1999 at the International Center for Insect Physiology and Ecology (ICIPE). The repellent is based on the potent phenolic analogue of a mild natural repellent of Savannah tsetse fly species. This single- component synthetic compound was discovered from a structure-activity study of the phenolic constituents of body odors of tsetse bovid hosts and their aged urine. Preliminary field trials undertaken among the pastoralist community in Nguruman Kenya, showed that the repellent caused more than 80% reduction in tsetse challenge and reduced the feeding efficiency of the tsetse fly on cattle by over 90%. This research technology needs to be packaged, ready for release and transfer to livestock farmers in tsetse fly infested areas. No chemical agent is entirely safe and likewise no chemical agent should be considered as being entirely harmfuL This concept is based on the premise that any chemical can be permitted to come into contact with a biologic mechanism without producing an effect on that mechanism provided the concentration of the chemical is below the toxic leveL This study was therefore undertaken to generate data on acute toxicity of the repellent in laboratory mice and effects of prolonged exposure to vapours of the repellent in goats as a prelude to its potential utilization for tsetse fly control in livestock. Data obtained from the acute toxicity studies in mice may be extrapolated to reflect the toxicity of the repellent to domestic animals and humans. In another experiment, rabbits were used to determine the ocular and dermal irritant effects of the repellent. Haematological and biochemical effects of the repellent were determined in goats exposed to low -2mg/hr and high doses -4mg/hr of the vapours of the repellent through repellent collars for nine months. After the nine months of exposure to the repellent, two goats from the low dose, high dose and the control group each were sacrificed and the chronic pathologic effects of the repellent determined using standard histopathology techniques. The 24 hours intraperitoneal (i.p) median lethal dose (LD5o) of the repellent in mice was found to be 40.2mg/kg body weight. The clinical signs of acute toxicity were decreased locomotor activity, an initial increase followed by a decrease in respiratory rate and an increased depth in respiration, terminal convulsions, coma and death. Death was probably due to depression and paralysis of the respiratory center in the brain. The repellent caused mild dermal irritation characterized by edema and erythema. There was moderate eye irritation affecting the cornea, iris and conjunctiva. Plasma levels of gamma glutamyl transpeptidase (GGT), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) were monitored monthly for nine months. There were no significant differences between the treated and the control animals in the plasma levels of the enzymes over the experimental period (p >0.05). For hematological parameters, the monthly means in all the groups for the RBC count were within the normal range for the species. There were also no significant differences between the means of the three groups (p>0.05). There was variation in the mean monthly values of the granulocyte and lymphocyte count that was not associated with the treatment since the trend was similar in both the treated and the control groups. The monthly group means for the WBC count were above the reference values for the species in all the groups including the pre-treatment values. There were no significant differences in the monthly means of the WBC count between the treated and the control group (p>0.05). Similarly, there was no significant difference in mean body weight between the treated and the control groups of the goats (p> 0.05). Histological sections prepared from the lungs, liver, kidney and heart tissues showed normal cellular and organ structure except for mild lymphocyte infiltration in the lung tissue in all the groups. According to the standard scheme for classifying relative toxicities, the LD50 in mice indicates that the synthetic repellent is highly toxic, manifesting central nervous system (CNS) effects. Ingestion of the repellent should, therefore, be avoided. The repellent is a mild skin irritant and causes eye irritation. However, the vapours of the repellent caused no effects on haematological and biochemical parameters and general health of exposed goats over the experimental period. Although the reactions in mice, rabbits and goats may not necessarily be the same as in humans, from the results, it is recommended that the tsetse fly repellent should be handled properly to avoid skin and eye contacts of the animal and the.handlers. Clear information on the toxicity should be availed to the animal handlers. Any accidental contact would be treated symptomatically.