dc.contributor.author | Makau, Mbithi | |
dc.date.accessioned | 2013-05-24T09:36:10Z | |
dc.date.available | 2013-05-24T09:36:10Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | Master of Medicine in Pathology, University of Nairobi, 2008 | en |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/11295/25326 | |
dc.description.abstract | Background: Gliomas are the most common primary central nervous
systemtumors, accounting for 48% of primary brain neoplasms. These are
tumours arising from glial cells in the brain namely astrocytes,
oligodendrocytes and ependymal cells all grouped into 24 tumour variants
in the World Health Organization 2000 classitication
Broad objective: To carry out immunohistochemical studies on all
gliomas, and classify them according to the WHO grading system 2000 and
correlate these with surgical and radiological diagnosis.
Design: A retrospective cross-sectional study.
Study subjects
All 102 patients operated on and diagnosed as having gliomas at KNH over
a period of five years from January 2002 to December 2006.
Setting: Kenyatta National Hospital histopathology laboratory and Kenya
Medical Research Institute- Mbagathi Nairobi.
Methods: Patients' data and histological reports were analyzed in order
to identify those who had been diagnosed to have gliomas. Patients
, diagnosed with gliomas during the study period were included. The
patients' surgical and radiological diagnoses were retrieved from their files.
A proforma form was used to key in patients' clinical and radiologic
information. Histology was done using paraffin embedded blocks, which
were retrieved from the library using the backup reports in the laboratory
library. Routine HIE staininq was done first, then immunohistochemical
markers, glial fibrillary acid protein (GFAP), and Ki67 a cellular proliferative
marker for grading.
The data collected was entered into spreadsheets in the computer and
processed using statistical package for social sciences. The results were
then presented in form of charts, tables and figures.
Results: The most prevalent glioma analyzed was grade I (pilocytic)
astrocytoma at 25%, followed by glioblastoma multiforme at 17.8%.
Majority of the patients were male at 57%. High-grade lesions, grades III
and IV gliomas were common among patients above 15 years and also
amongst male patients.
Correlations between radiological, surgical and histological diagnosis was
very poor at 16%.
All gliomas analyzed were reactive to GFAP and ki67 staining was useful in
grading of the tumours and in differentiating low-grade astrocytomas from
gliosis. The project was carried out over a period of one year.
Conclusion: The use of Immunohistochemistry is usefulin the routine
histological diagnosis and grading of gliomas at Kenyatta national hospital
and the need for complete clinical and radiological detail of the patient is
important for correct histological interpretation.
We recommend that a standard proforma or request form be developed
and adhered to for reporting brain tumours and that GFAP and ki67
Immunohistochemical panels be introduced in the laboratory for diagnosis
of gliomas. | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi. | en |
dc.title | Gliomas: Immunohistochemical Grading And Clinico-pathologic Correlations At Kenyatta National Hospital | en |
dc.type | Thesis | en |
dc.description.department | a
Department of Psychiatry, University of Nairobi, ; bDepartment of Mental Health, School of Medicine,
Moi University, Eldoret, Kenya | |
local.publisher | Department Human Pathology | en |