In vitro embryo culture of interspecific hybrids between the kenyan lines of phaseolus vulgariis L. and phaseolus acutifolius A. gray
Abstract
The objective of this study was to examine the
interspecific compatibility between the Kenyan
Phaeseolus vulgaris L. lines (GLP 2, GLP 24, GLP X92
GLP 1004 and 1122) and Phae eo Lu e a cu t i f o l iu e Ao Gray
lines (P. I. 310801, P.I.310606, P.I 263590, L-246-2,
L-179, L-170, L-242-2, L-246-l2, L-242-6, CIAT G400l6,
CIAT G40034, Buff Hood Arizona and White Hood Arizona)
with the hope for future exchange of desirable genetic
characteristics between the two species.
Pre-mature abortion of hybrid embryos obtained
from several crosses between the two species was
successfully overcome by in vitro embryo culture.
Rooting was poor among interspecific hybrids. N6_
benzyl adenine promoted shoot development but retarded
rooting. Inclusion of both kinetin and indole-acetic
acid in the basal medium did not significantly improve
root development. However, root development was
promoted by either excluding growth hormones from
the basal medium or by using certain genotype combinations
of various lines of Phaseolus vulgaris L. and
Phaseolus acutifolius.
xxiv
Mature interspecific hybrids were produced
between: 'GLP 24 and Buff Hood Arizona', GLP 1004
and CIAT G400161
, '1122 and P.L 310801'. The
hybrids expressed various foliar morphological and
growth characteristics which were overdo minant or
intermediate between the parents. Two Fl hybrids
(GLP 24 X Buff Hood Arizona) expressed leaf morphological
characteristics e.g. acute leaf apices and
indeterminate growth habit closely resembling
'Buff Hood Ar i z o na ", The Fl hybrid ('GLP 1004 X
CIAT G400161
) had acute leaf apices and indeterminate
growth habit of ICIAT G400l61
, while other
leaf characteristics eog. widths of the leaf lamina
of terminal and lateral leaflets closely resembled
'GLP 10041
, The F1 hybrid (11122 X Pol. 3108011
)
had indeterminate growth habit of I Po 10 3108011
while leaf characteristics eogo rounded leaf bases
of terminal leaflets closely resembled 6112260
Citation
A thesis submitted in partial fulfilment of the requirements of the degree of master of science in plant breedingPublisher
Plant Science & Crop Protection, University of Nairobi