Dried blood spot HIV-1 RNA quantification using open real-time systems in South Africa and Burkina Faso
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Date
2010-11Author
Viljoen, J
Gampini, S
Danaviah, S
Valéa, D
Pillay, S
Kania, D
Méda, N
Newell, ML
Van de Perre, P
Rouet, F
World Health Organization/ANRS 1289 Kesho Bora Study Group
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
There is an urgent need to assess the accuracy/feasibility of using dried blood spots (DBS) for monitoring of HIV-1 viral load in resource-limited settings. A total of 892 DBS from HIV-1-positive pregnant women and their neonates enrolled in the Kesho Bora prevention of mother-to-child transmission trial conducted in Durban (South Africa) and Bobo-Dioulasso (Burkina Faso) between May 2005 and July 2008 were tested for HIV-1 RNA. The combination Nuclisens extraction method (BioMérieux)/Generic HIV Viral Load assay (Biocentric) was performed using one DBS (in Durban) versus 2 DBS (in Bobo-Dioulasso) on 2 distinct open real-time polymerase chain reaction instruments. DBS HIV-1 RNA results were compared with plasma HIV-1 RNA and HIV serology results used as the gold standards. The limits of detection of assays on DBS were 3100 and 1550 copies per milliliter in Durban and Bobo-Dioulasso, respectively. DBS HIV-1 RNA values correlated significantly with plasma levels (n = 327; R = 0.7351) and were uniformly distributed according to duration of DBS storage at -20°C (median duration, 280 days). For early infant diagnosis, the sensitivity and specificity were 100% (95% confidence interval: 97.2 to 100.0 and 96.5 to 100.0, respectively). HIV-1 viral load kinetics in DNase-pretreated DBS were similar to those obtained in plasma specimens among 13 patients receiving antiretroviral treatment. HIV-1 RNA findings from serial infant DBS collected prospectively (n = 164) showed 100% concordance with HIV serology at 18 months of life. Our findings strongly advocate the implementation of DBS HIV-1 RNA testing in remote areas from low-income and middle-income countries.
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http://www.ncbi.nlm.nih.gov/pubmed/20700058http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/32186
Citation
J Acquir Immune Defic Syndr. 2010 Nov;55(3):290-8. doi: 10.1097/QAI.0b013e3181edaaf5Publisher
University of Nairobi School of medicine,University of Nairobi
Collections
- Faculty of Health Sciences (FHS) [10378]