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    High-Level Heterologous Expression of Bacillus halodurans Putative Xylanase Xyn11A (BH0899) in Kluyveromyces lacti

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    Date
    2007-03
    Author
    Wamalwa Benson Munyali.
    Zhao Guangshan.
    Sakka Makiko.
    Shiundu Paul Mwanza.
    Kimura Tetsuya.
    Sakka Kazuo.
    Type
    Article
    Language
    en
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    Abstract
    The putative xyn11A structural gene (BH0899) encoding a family-11 xylanase from alkaliphilic Bacillus halodurans strain C-125 was heterologously expressed in the yeast Kluyveromyces lactis CBS 1065 and secreted to a level of 156 μg/ml under selective culture conditions in shake flasks. The Xyn11A production level in shake flask cultures of K. lactis CBS 1065 was higher than that reported for other xylanase genes placed under the control of the regulated LAC4 promoter on a plasmid containing an entire sequence of pKD1 from Kluyveromyces drosophilarium. Recombinant Xyn11A was highly active over pH range from 3 to 10, with maximal activity around pH 7. The enzyme showed a specific activity of 628 U/mg-protein on birchwood xylan as substrate, but no cellulase or β-xylosidase activity.
    URI
    https://www.jstage.jst.go.jp/article/bbb/71/3/71_60477/_article
    http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/33851
    http://www.ncbi.nlm.nih.gov/pubmed/17341817
    Citation
    Bioscience, Biotechnology, and Biochemistry Vol. 71 (2007) No. 3 P 688-693
    Publisher
    Department of Chemistry, University of Nairobi
    Collections
    • Faculty of Science & Technology (FST) [4284]

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