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    HPCL Separation and Quantitative Determination of Valepotriates from Valeriana kilimandascharica

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    Date
    1981
    Type
    Article
    Language
    en
    Metadata
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    Abstract
    Valepotriates, mainly isovaltrate and valtrate, have been separated and quantitatively estimated by reversed-phase HPLC in the leaves, flowers, stems and rhizomes of Valeriana kilimandascharica. The isovaltrate/valtrate concentration reaches a maximum of 5.89% in the leaves, 3.84% in the flowers, 3.17% in the stems and 5.15% in the rhizomes. A µ Bondapak C18 column using MeOH-H2O mixtures as eluant is suitable for a baseline separation of isovaltrate, valtrate, acevaltrate and baldrinal at UV 254 nm in 15 min and didrovaltrate and IVHD-valtrat at UV 208 nm in 10 min. Relative standard deviation for quantitative determinations is approximately 1.5% for valepotriate contents of 1%. This method is adaptable for routine analysis of crude extracts.
    URI
    https://www.thieme-connect.com/ejournals/abstract/10.1055/s-2007-971496
    http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/35085
    Citation
    lanta Med 1981; 43(10): 179-182 DOI: 10.1055/s-2007-971496
    Publisher
    Department of botany, University of Nairobi
    Subject
    Valeriana
    Valerianaceae
    Valepotriates
    HPLC
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    • Faculty of Science & Technology (FST) [4284]

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