Evidence for two single copy units in Theileria parva ribosomal RNA genes.
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Date
1994Author
Kibe, MK
ole-MoiYoi, OK
Nene, V
Khan, B
Allsopp, BA
Collins, NE
Morzaria, SP
Gobright, EI
Bishop, RP
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Bacteriophage clones containing ribosomal RNA genes of Theileria parva were isolated from genomic DNA libraries. Physical mapping studies revealed 2 ribosomal DNA units, which were distinguishable by restriction enzyme site polymorphisms in flanking sequences. The cloned ribosomal DNA units were mapped to 2 separate T. parva chromosomes. Analysis of sequences contained in lambda EMBL3 recombinants, together with Southern blot analysis of genomic DNA and data on the copy number of the rRNA genes, suggested that the rDNA units were not tandemly repeated. This organisation of ribosomal transcription units is similar to that described for other genera of apicomplexan protozoa, but 2 rDNA units, each containing single copies of the rRNA coding genes, would be the lowest copy number described for any eukaryote in which amplification of rRNA genes is not known to occur. EcoRI restriction fragment length polymorphisms, which were revealed using rRNA gene probes, separated T. parva stocks into 2 categories. Nucleotide sequence analysis of polymerase chain reaction-amplified internal transcribed spacer DNA revealed 2 different ITS sequences derived from rDNA transcription units within the genome of a cloned T. parva parasite. Polymorphism was also observed between ITS sequences amplified from the DNA of different T. parva stocks. A synthetic oligonucleotide derived from T. parva Uganda ribosomal ITS DNA sequences hybridised to DNA from the T. parva Uganda stock, but not to the DNA of the T. parva Muguga stock. This oligonucleotide is potentially useful as a marker for the T. parva Uganda stock
URI
http://hinari-gw.who.int/whalecomwww.ncbi.nlm.nih.gov/whalecom0/pubmed/7808475http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/36383
Citation
Mol Biochem Parasitol. 1994 Aug;66(2):249-59Publisher
International Laboratory for Research on Animal Diseases (ILRAD), Nairobi, Kenya. College of Health Sciences, University of Nairobi
Collections
- Faculty of Health Sciences (FHS) [10378]