Partial characterization and post‐feeding activity of midgut aminopeptidase in the human body louse, Pediculus humanus humanus
Date
1998Author
Ochanda, James O
Oduor, Eva A. C
Galun, Rachel
Imbuga, Mabel O
Mumcuoglu, Kosta Y
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
A leucine aminopeptidase was found in the midgut of the human body louse, Pediculus humanus humanus L. (Anoplura: Pediculidae). The enzyme is activated by the bloodmeal with a pH optimum at 8. The enzyme is soluble in both aqueous and detergent-containing solutions. The two forms of the enzyme had the same Km but exhibited different catalytic activities with regard to Vmax values in these solutions. The enzyme is inhibited competitively by a substrate analogue 1,10-phenanthroline and by Mn2+ ions in the presence and absence of detergent.
URI
http://onlinelibrary.wiley.com/doi/10.1046/j.1365-3032.1998.234096.x/abstracthttp://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/36425