| dc.contributor.author | Ogoyia, Dorington O | |
| dc.contributor.author | Ochanda, James, O | |
| dc.contributor.author | Osirac, Ellie, O. | |
| dc.date.accessioned | 2013-06-26T07:16:50Z | |
| dc.date.available | 2013-06-26T07:16:50Z | |
| dc.date.issued | 1993 | |
| dc.identifier.citation | OGOY11, D. O., OSIR, E. O., & Ochanda, J. O. (1993). Lipophorin of the larval stalk borer, Busseola fusca: Purification and properties. | en |
| dc.identifier.uri | http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=7463372 | |
| dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/40180 | |
| dc.description.abstract | Lipophorin was isolated from the stalk borer, Busseolafusca, larvae by ultracentrifugation in a KBr density-gradient. The lipophorin (M, ˜ 700,000) is a high density lipoprotein (density = 1.13 g/ml) composed of 46% lipids and 4% carbohydrates. It consists of two apoproteins, apolipophorin-I (apo Lp-I, Mr ˜210,000) and apolipophorin-II (apo Lp-II, Mr ˜78,000). Both apoproteins are glycosylated as shown by periodate Schiff reagent staining and binding to the lectin, concanavalin A. Amino acid composition analysis of lipophorin showed predominance of aspartate (13%), glutamate (9%) and glycine (9%).
In an immunoblotting experiment, both apoproteins showed immunoreactivity with antiserum to B, fusca lipophorin and no cross-reactivity occurred with any other haemolvmph proteins. Immunodiffusion studies with the same antibodies showed cross-reactivity with haemolymph derived from other Lepidoptera and not from Orthoptera, Dictyoptera or Diptera. | en |
| dc.language.iso | en | en |
| dc.publisher | University of Nairobi | en |
| dc.title | Lipophorin Of The Larval Stalk Borer, Busseola Fusca: Purification And Properties | en |
| dc.type | Article | en |
| local.publisher | Department of Biochemistry, University of Nairobi, Nairobi, | en |
