Studies on Nitrate Reductase and Nitrate Reductase Inhibiting Enzyme in field-grown finger millet (Eleusine coracana (L.) Gaertn.

Abstract

The induction of NRA by nitrate has been demonstrated in various plants under strictly controlled conditions. When nitrate supply is limited, NRA is almost undetectable in the majority of the plants. This has led to the conclusion that nitrate is the main inducer of NRA. On the other hand, NRIE activity has not been shown to respond similarly towards nitrate. The conclusion has therefore been that NRIE is essentially a constitutive enzyme. In this study, the influence of nitrogenous fertiliser on NR and NRIE activities was investigated. Nitrate reductase and nitrate reductase inhibiting enzyme activities were assayed both from leaf and root tissues.