Liquid chromatographic analysis of phenobarbitone, ethosuximide, phenytoin and carbamazepine on a polystyrene-divinyl benzene column
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Date
2005Author
Amugune, BK
Thoithi, GN
Kibwage, IO
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
A liquid chromatographic method for the simultaneous assay of four anticonvulsant drugs, phenobarbitone, ethosuximide, phenytoin and carbamazepine on a polystyrene-divinyl benzene column is described. The method was developed by the systematic study of different types of co-polymer materials, type and concentration of organic modifiers, buffer pH and concentration and column temperature. A PLRP-S 100 Å 8 µm column maintained at 60 oC and a mobile phase consisting of acetonitrile-tert-butanol-phosphate buffer (pH 7.6, 0.2 M)-water (25:5:10:60, v/v) were used. The flow rate was 1 ml/min with ultraviolet detection at 220 nm. The method has been validated and used for the analysis of raw materials, finished products and dissolution studies of the drugs.
URI
http://www.ajol.info/index.php/ecajps/article/view/9732http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/40779
Citation
The East and Central African Journal of Pharmaceutical Sciences Vol. 8 (3) 2005: pp 19-25Publisher
University of Nairobi College of Health Sciences,University of Nairobi
Subject
Liquid chromatography, co-polymer column, phenobarbitone, ethosuximide, phenytoin, carbamazepine.Collections
- Faculty of Health Sciences (FHS) [10378]