dc.contributor.author | Luo, M | |
dc.contributor.author | Capina, R | |
dc.contributor.author | Daniuk, C | |
dc.contributor.author | Tuff, J | |
dc.contributor.author | Peters, H | |
dc.contributor.author | Kimani, M | |
dc.contributor.author | Wachihi, C | |
dc.contributor.author | Kimani, J | |
dc.contributor.author | Ball, T B | |
dc.contributor.author | Plummer, F A. | |
dc.date.accessioned | 2013-06-27T14:41:51Z | |
dc.date.available | 2013-06-27T14:41:51Z | |
dc.date.issued | 2013-06 | |
dc.identifier.citation | Vaccine. 2013 Jun 24;31(29):3000-8. doi: 10.1016/j.vaccine.2013.04.057. Epub 2013 May 10. | en |
dc.identifier.uri | http://hinari-gw.who.int/whalecomwww.ncbi.nlm.nih.gov/whalecom0/pubmed/23664989 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/41270 | |
dc.description.abstract | Developing an effective preventative vaccine against HIV-1 has proved to be a great challenge. The classical and proven vaccine approach has failed so far or produced a modest effect, new approaches are needed. In this study we evaluated the immunogenicity of the sequences around the protease cleavage sites (PCS) and the population coverage of a vaccine targeting HIV-1 PCS. The sequence conservation was evaluated by comparing entropy score of sequences around PCS with Gag and Pol. The immunogenicity of sequences around the 12 PCS (+10/-10 amino acids) was analyzed by identifying epitopes of HLA class I alleles in PCS region using four approaches: (1) identification of previously reported HLA class I allele epitopes around PCS region; (2) screening and validating epitopes of 8 HLA class I alleles common to most world populations using iTopia Epitope Discovery system and IFN-γ ELISpot assays; (3) screening of 151 patients of Pumwani cohort for PBMC IFN-γ ELISPOT responses to the subtype A and D consensus around PCS region; and (4) prediction of HLA alleles with epitopes around the PCS using NetMHCpan. Population coverage was calculated using the web-based analysis tool of the Immune Epitope Database based on HLA class I genotype frequencies from dbMHC database. The results showed that many HLA class I alleles have multiple epitopes in the 12 PCS regions, indicating sequence immunogenicity around PCS. Multiple epitopes of many HLA class I alleles common to >95% world populations have been identified around the 12 PCS region. Targeting these sites is a feasible vaccine approach. | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi | en |
dc.title | Immunogenicity Of Sequences Around Hiv-1 Protease Cleavage Sites: Potential Targets And Population Coverage Analysis For A Hiv Vaccine Targeting Protease Cleavage Sites. | en |
dc.type | Article | en |
local.publisher | Department of Medical Microbiology | en |