Hormonal control of strawberry axillary bud development in vitro.

Abstract

Axillary bud and stolon tip explants of strawberry cultured on Murashige and Skoog basal media containing kinetin at 1, 5 or 10 mg/l developed into leafy shoots. A low concentration of kinetin (1 mg/l) promoted the development of both types of explant into single shoots while a higher concentration (10 mg/l) promoted production of multiple leafy shoots. NAA at 1 mg/l promoted callus growth from both types of explant. Axillary bud explants developed into stolons when cultured on media containing GA3 at 5, 10 or 20 mg/l. Stolon apices developed into leafy shoots. Combinations of GA3 and kinetin induced the development of axillary bud explants into structures intermediate between stolons and leafy shoots. Stolon apices and stolon axillary buds developed into leafy shoots and stolons, respectively, when cultured on a kinetin-containing medium for one week, and then transferred onto a GA3-containing medium. Thus, the developmental pathway of axillary strawberry buds was shown to be responsive to a balance between GA and cytokinins following removal from apical dominance