Plasmodium falciparum genotyping by microsatellites as a method to distinguish between recrudescent and new infections
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Date
2005Author
D’alessandro, Umberto
Dujardin, Jean-Claude
Laurent, Thierry
Overmeir, Chantal Van
Nyachieo, Atunga
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
In vivo tests for susceptibility to antimalarial drugs require molecular methods to distinguish recrudescence
from new infection. The most commonly used DNA markers (merozoite surface proteins [MSPs]) are under immune
selective pressure, which might lead to misclassification. We evaluated immunologically neutral microsatellite markers
in blood samples collected during a drug efficacy trial in Rwanda. Fifty percent of the infections classified as recrudescent
by MSP were classified as new by microsatellite markers. Reciprocally, 23.3% of infections classified as recrudescent by
microsatellite markers were identified as new by MSP. In drug efficacy studies, microsatellite markers should complement
MSP genotyping to distinguish a recrudescence from a new infection
Citation
The American Society of Tropical Medicine and Hygiene 73(1), 2005, pp. 210–213Publisher
Department of Biochemistry
Collections
- Faculty of Health Sciences (FHS) [10378]