dc.contributor.author | Mulli Tonnie K. | |
dc.contributor.author | Hughes, FJ | |
dc.contributor.author | Mckay, IJ | |
dc.date.accessioned | 2013-10-24T08:31:38Z | |
dc.date.available | 2013-10-24T08:31:38Z | |
dc.date.issued | 2010-07 | |
dc.identifier.citation | TK Mulli; FJ Hughes; IJ Mckay, Salivary Antimicrobial Peptides As Potential Biomarkers Of Periodontal Disease, 2010 | en |
dc.identifier.uri | https://iadr.confex.com/iadr/2010barce/preliminaryprogram/abstract_130783.htm | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/57868 | |
dc.description.abstract | Objectives: Using SELDI-TOF-MS we have previously identified putative salivary biomarkers of periodontitis. Some of these identified peaks were predicted to be antimicrobial peptides (AMPs), specifically alpha defensins 1-3 (HNP 1-3) and cathelicidin (LL-37). AMPs are cationic peptides produced by neutrophils and are important in innate and adaptive immunity. Thus the aim of this study was to investigate further salivary HNP 1-3 and LL-37 concentrations as potential diagnostic biomarkers for gingivitis (G), chronic periodontitis (CP) and aggressive periodontitis (AgP). Methods: WS was collected using salivettes in subjects with G (n=18), CP (n=22) and AgP (n=20) and measured using SELDI-TOF-MS. We also tested the effect of non-surgical therapy in an independent AgP cohort (n=47). ELISAs for HNP1-3 and LL-37 were carried out to confirm and quantify the data. Difference in expression levels were analyzed using ANOVA and Bonferoni's correction (P < 0.05). Results: HNP1-3 and LL-37 were first identified by SELDI-TOF-MS and LC-MS/MS confirmed their identity. Using ELISAs, HNP 1-3 levels were significantly higher in CP than in G (p=0.01; 0.7µg/ml and 0.2µg/ml respectively) and before treatment than after treatment (p=0.045; 0.92µg/ml and 0.41µg/ml respectively). LL-37 levels were significantly higher in AgP than in both G & CP (p=0.007 & p=0.013 respectively) with100% sensitivity and specificity for AgP vs G (p=0.0003) and 100% sensitivity and 81.8% specificity for AgP vs CP (p=0.002). Conclusion: Salivary HNP1-3 and LL-37 can differentiate G, CP & AgP with high specificity and sensitivity. Further investigations in larger independent cohorts will assess how useful these AMPs are in discriminating periodontal disease status. | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi | en |
dc.title | Salivary Antimicrobial Peptides As Potential Biomarkers Of Periodontal Disease | en |
dc.type | Article | en |
local.publisher | School of Dental Sciences | en |