| dc.contributor.author | Onyango, Noel O | |
| dc.contributor.author | Muchugi, Alice | |
| dc.contributor.author | Muge, Edward | |
| dc.contributor.author | Aman, Bonaventure O | |
| dc.contributor.author | Jamnadass, Ramni | |
| dc.date.accessioned | 2014-02-28T11:50:52Z | |
| dc.date.available | 2014-02-28T11:50:52Z | |
| dc.date.issued | 2013 | |
| dc.identifier.citation | Ochieng ON, Muchugi A, Muge E, Aman BO, Jamnadass R. "Identification of Specific Markers Linked to Regional Differentiation of Warburgia ugandensis." Journal of Life Sciences. 2013;5(1):29-40. | en_US |
| dc.identifier.uri | http://hdl.handle.net/11295/65023 | |
| dc.identifier.uri | http://www.krepublishers.com/02-Journals/JLS/JLS-05-0-000-13-Web/JLS-05-1-000-13-Abst-PDF/JLS-05-1-029-13-139-Ochieng-O-N/JLS-05-1-029-13-139-Ochieng-O-N-Tt.pdf | |
| dc.description.abstract | Warburgia ugandensis is an important African medicinal tree. The species population has shown a high genetic differentiation in the Kenya’s Rift Valley. Nine populations were analysed by Bulk Segregant Analysis employing Random
Amplified Polymorphic DNA marker technique to identify regional differentiation-linked markers within and across Kenyan
Rift Valley. Five primers showed putative East and West genetic differentiation. Diagnostic markers were isolated, cloned,
sequenced and compared with Genbank sequences using BLAST algorithms. Three, (WarburgiaIC15E, WarburgiaIC55E and
WarburgiaIC28W) sequences showed homology to plant and bacterial-like chromosomal sequences with low E-values. Sequence
alignment indicated conserved protein domains of plants and bacteria-like sequences. Phylogenetic analysis revealed high rates
of genetic distances (H” 0.8) and a low rate of disparity indices of (0), suggesting some evolutionary forces behind demographic
differentiation. These imply that genetic differentiation observed might be due to genetic mutants in certain domains of
chromosome that may have some implication on genome functionalit | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | University of Nairobi | en_US |
| dc.subject | Random Amplified Polymorphic DNA. Amplified Fragment Length Polymorphism. Basic Local Alignment Search Tool. Multiple Sequence Alignment. Sequence | en_US |
| dc.title | Identification of specific markers linked to regional differentiation of Warburgia ugandensis | en_US |
| dc.type | Article | en_US |
