Multiple Plantlets In Lateral Bud And Leaf Explant In Vitro Cultures Of Pineapple
Date
1979-12Author
Mathews, V Helena
Rangan, TS
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Plantlets were obtained from usually dormant axillary buds, excised from the crown of pineapple (Ananas comosus L. Merr.) and grown in culture. Multiple shoots arose from single buds grown on Murashige and Skoog medium supplemented with auxins and kinetin. Shaking culture-flasks during growth increased the number of multiple shoots formed, when compared with stationary liquid cultures. Leaf explants excised from in vitro plantlets developed into a callus capable of plantlet regeneration. Subjecting developing buds to surgical segmentation also resulted in multiple shoot formation. Such shoots, when excised and grown on Murashige and Skoog medium supplemented with auxins, developed roots and grew into complete plantlets capable of being grown in soil.
URI
http://www.sciencedirect.com/science/article/pii/0304423879900165http://hdl.handle.net/11295/66406
Citation
Scientia Horticulturae Volume 11, Issue 4, December 1979, Pages 319–328Publisher
University of Nairobi