Browsing Faculty of Agriculture & Veterinary Medicine (FAg / FVM) by Author "Igarashi, I"
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Alternative methods in laboratory diagnosis of equine piroplasmosis.
Karanja, DN; Yeboah, RK; Yokoyama, N; Igarashi, I (University of Nairobi,, 2005)In vitro culture, spectrophotometry, agarose gel electrophoresis and polymerase chain reaction were used to detect Babesia equi parasites. A ten-fold serial dilution of a 10% parasitemic culture was done to simulate low ... -
C3 contributes to the cross-protective immunity induced by Babesia gibsoni phosphoribo protein P0 against a lethal B. rodhaini infection
Terkawi, M A; Zhang, G; Jia, H; Aboge, G; Goo, Y K; Nishikawa, Y; Igarashi, I (Department of Public Health Pharmacology and Toxicology, 2008)We have studied the impact of complement component 3 (C3) deficiency on the progression of lethal Babesia rodhaini infection in immune mice. A B. gibsoni ribosomal phosphoriboprote in P0 (BgP0) previously reported to be ... -
C3 contributes to the cross-protective immunity induced by Babesia gibsoni phosphoribo protein P0 against a lethalB. rodhaini infection
Terkawi, M A; Zhang, G; Jia, H; Aboge, G; Goo, Y K; Nishikawa, Y; Nishikawa, Y; Igarashi, I (Department of Public Health Pharmacology and Toxicology, 2008)We have studied the impact of complement component 3 (C3) deficiency on the progression of lethal Babesia rodhaini infection in immune mice. A B. gibsoni ribosomal phosphoriboprote in P0 (BgP0) previously reported to be ... -
Characterization of a leucine aminopeptidase of Babesia gibsoni
Jia, H; Terkawi, M. A; Aboge, G. O; Goo, Y.K; Luo, Y; Li, Y; Yamagishi, J; Nishikawa, Y; Igarashi, I; Sugimoto, C; Fujisaki, K; Xuan, X (2009)Peptidases of parasitic protozoa are currently under intense investigation in order to identify novel virulence factors, drug targets, and vaccine candidates, except in Babesia. Leucine aminopeptidases in protozoa, such ... -
Identification and characterization of an interspersed repeat antigen of Babesia microti (BmIRA)
Cao, S; Luo, Y; Aboge, GO; Terkawi, MA; Masatani, T; Suzuki, H; Igarashi, I; Nishikawa, Y; Xuan, X (2013)In this report, a novel gene encoding an interspersed repeat antigen from Babesia microti (BmIRA) was identified and described. The full-length cDNA containing an open reading frame of 1,947 bp was obtained by immunoscreening ... -
Identification of an immunodominant Babesia gibsoni 47-kDa antigen
Goo, Y-K; Jia, H; Aboge, G O; Terkawi, M A; Yamagishi, J; Nishikawa, Y; Igarashi, I; Xuan, X (Department of Public Health, Pharmacology and Toxicology, 2009)The inosine monophosphate dehydrogenase(IMPDH )enzyme has been characterized and validate dasa molecular drug target in other apicomplexans butnot in the genus Babesia. Subsequently,we cloned and expressed a Babesia ... -
Molecular and immunological characterization of Babesia gibsoni
Terkawi, M Alaa; Aboge, G; Jia, H; Goo, Y-K; Ooka, H; Yamagishi, J; Nishikawa, Y; Yokoyama, N; Igarashi, I; Kawazu, S-I; Fujisaki, K; Xuan, X (Department of Public Health, Pharmacology and Toxicology, 2009)Serological immune screening was used to identify a gene encoding heat shock protein-70 from Babesia gibsoni (BgHSP-70) that showed high homology with HSP-70s from other apicomplexan parasites. This gene corresponded to a ... -
Molecular characterization of a novel 32-kDa merozoite antigen of Babesia gibsoni with a better diagnostic performance by enzyme-linked immunosorbent assay
Aboge, G O; Jia, H; Kuriki, H; Zhou, J; Nishikawa, Y; Igarashi, I; Fujisaki, K; Suzuki, H; Xuan, X (Department of Public Health, Pharmacology and Toxicology, 2007)We cloned and expressed 3 novel gene encoding a 32-kDa merozoite protein of Babesia gibsoni (BgP32). The length of nucleotide sequence of the cD;\' A \\'3. 1-1-6-1 bp with an open reading frame of 969 bp. The truncated ... -
Molecularand immunological characterization of Babesia gibsoni and Babesia microti heat shockprotein-70
Terkawi, M A; Jia, H J; Goo, Y-K; Ooka, H; Yamagishi, J; Nishikawa, Y.; Igarashi, I; Fujisaki, K; Xuan, X (Department of Public Health Pharmacology and Toxicology, 2009)Serological immunoscreening was used to identify a gene encoding heat shock protein-70 from Babesia gibsoni (BgHSP-70) that showed high homology with HSP-70s from other apicomplexan parasites. This gene corresponded to ... -
Seroprevalence of Cryptosporidium parvum infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23
Inpankaew, T; Jittapalapong, S; Phasuk, J; Pinyopanuwut, N; Chimnoi, W; Kengradomkit, C; Sunanta, C; Zhang, G; Aboge, G.O; Nishikawa, Y; Igarashi, I; Xuan, X (2009)Cryptosporidium parvum is the most frequent parasitic agent that causes diarrhoea in AIDS patients in Thailand. Cryptosporidiosis outbreaks in humans may be attributed to contamination of their drinking water from infected ...