Reserpine as an uncoupling agent
Abstract
1. Reserpine, like the uncoupling agent, 2,4-dinitrophenol prevents oxidative phosphorylation but stimulates the rate at which oxygen is reduced.
2. Both reserpine and 2,4-dinitrophenol fail to stimulate oxygen uptake by isolated mitochondria in the presence of arginine.
3. Both 2,4-dinitrophenol and reserpine induce proton permeability in the mitochondrial membrane so that H+ is absorbed from the suspending medium.
4. When the reaction system contains reserpine, accumulation of Ca2+ by mitochondria is inhibited.
5. Reserpine decreases both ADP:O and P:O ratios which strongly suggest that reserpine is an uncoupling agent.
URI
http://www.sciencedirect.com/science/article/pii/0005272874901327http://hdl.handle.net/11295/91402
Citation
Biochimica et Biophysica Acta (BBA) - Bioenergetics Volume 333, Issue 3, 26 March 1974, Pages 481-486Publisher
University of Nairobi
Collections
- Faculty of Health Sciences (FHS) [10387]