Turbidimetric Procedure for Determination of Lipase Activity
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Date
1973-04Author
Burlina, Angelo
Galzigna, Lauro
Language
enMetadata
Show full item recordAbstract
A method is described for turbidimetric assay of lipase activity. The substrate is a two-phase emulsion composed of a fairly homogenous dispersion of micelles of either triolein or olive oil. The de-emulsification of the substrate after lipase addition is a true measure of the enzymatic activity. Turbidity change is related to amount of the enzyme by use of a commercial standard, purified lipase from hog pancreas. The quantitative relationship between turbidity change and the number of micelles provides the theoretical basis for this type of assay.
Citation
Clinical Chemistry April 1973 vol. 19 no. 4 384-386Publisher
University of Nairobi
Collections
- Faculty of Health Sciences (FHS) [10387]